rabbit anti calbindin calb  (Swant)

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Deciphering Auditory Hyperexcitability in Otogl Mutant Mice Unravels an Auditory Neuropathy Mechanism.

doi: 10.1002/advs.202410776

Figure Lengend Snippet: Figure 4. Molecular characterization of tdTomato-positive neurons in Otogl IREScre/+:Rosa-tdTomato mice. A) Z-projections of the apical cochlear spiral ganglia of a P28 Otogl IREScre/+:Rosa-tdTomato mouse immunostained for PV, Pou4f1, calb, or CR, or stained for Prph mRNA (top) with enlarged views of the insets showing details of SGNs (bottom). B) Bar graphs showing the percentage of tdTomato-positive neurons positive for PV, the distribution of PV neurons positive for tdTomato at the apex, middle, and base of the cochlea, the percentage of tdTomato-positive neurons also positive for Pou4f1, calb, CR, or Prph mRNA, and the percentage of tdTomato-negative neurons positive for Pou4f1, calb, CR, or Prph mRNA. Cell nuclei are stained in blue (DAPI). Numbers in brackets indicate the number of neurons counted.

Article Snippet: Primary antibodies (anti-) Host species Dilution Supplier Reference Calbindin (calb) rabbit 1:1000 Cell Signaling 13176 Calretinin (CR) rabbit 1:1000 Swant 7697 CtBP2 mouse 1:2000 BD Biosciences 612044 DARPP-32 rabbit 1:1000 Abcam ab40801 GATA3 goat 1:50 Bio-Techne SAS AF2605 GluA2 mouse 1:500 Millipore MAB397 Alexa Fluor® 647 anti-Myelin basic protein SMI-99 (MBP) mouse 1:500 BioLegend 808408 Myosin7a (Myo7a) rabbit 1:100 Proteus 25-6790 NF200 chicken 1:1000 Millipore AB5539 Parvalbumin (PV) mouse 1:1000 Swant PV235 Pou4f1 (Brn-3a) mouse 1:50 Millipore MAB1585 RFP rabbit 1:1000 Bio Vision 3993-100 Syt2 (Znp-1) mouse 1:1000 ZIRC AB_10013783 pure tones (10–14 kHz) lasting up to 1 min, during the P21–P28 and P35– P40 periods.

Techniques: Staining

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Deciphering Auditory Hyperexcitability in Otogl Mutant Mice Unravels an Auditory Neuropathy Mechanism.

doi: 10.1002/advs.202410776

Figure Lengend Snippet: Figure 8. Elevated re-ABR thresholds in Otogl conditional knock-out mice. A) Z-projections of apical cochlear spiral ganglia cross-sections from a P27 Bhlhb5cre/+:Rosa-tdTomato mouse immunostained for PV, Pou4f1, calb, or CR (top) with enlarged views of the insets showing details of SGNs (bottom). Bar graphs showing the percentage of tdTomato-positive neurons also positive for PV (top) and the percentage of tdTomato-positive neurons also positive for Pou4f1, calb, or CR (bottom). Cell nuclei are stained in blue (DAPI). B) Example traces of ABR recordings from P25 control Bhlhb5+/+:Otogl lox/lox (top left) and Bhlhb5cre/+:Otogl lox/lox (top right) in response to 10 kHz pure tones at 85, 95, and 105 dB. Example traces of reABR recordings obtained from P25 Bhlhb5+/+:Otogl lox/lox (bottom left) and Bhlhb5cre/+:Otogl lox/lox (bottom right) mice, after increasing the 10 kHz probe tone by 10, 20, and 30, with masking of the classical ABR signal obtained in response to a 10 kHz probe tone at 75 dB with a ≈70 dB masker noise. The red asterisk indicates the almost total absence of a re-emerging wave I signal when the 10 kHz probe tone is increased by 10 dB. C) Bar graphs showing the wave I amplitude ratio (reABR/ABR) (top) and the wave V amplitude ratio (bottom) as a function of probe tone sound level increase in P25 Bhlhb5+/+:Otogl lox/lox and Bhlhb5cre/+:Otogl lox/lox mice. Numbers in brackets indicate either the number of probed neurons (A) or tested animals (C). ns, non-significant.

Article Snippet: Primary antibodies (anti-) Host species Dilution Supplier Reference Calbindin (calb) rabbit 1:1000 Cell Signaling 13176 Calretinin (CR) rabbit 1:1000 Swant 7697 CtBP2 mouse 1:2000 BD Biosciences 612044 DARPP-32 rabbit 1:1000 Abcam ab40801 GATA3 goat 1:50 Bio-Techne SAS AF2605 GluA2 mouse 1:500 Millipore MAB397 Alexa Fluor® 647 anti-Myelin basic protein SMI-99 (MBP) mouse 1:500 BioLegend 808408 Myosin7a (Myo7a) rabbit 1:100 Proteus 25-6790 NF200 chicken 1:1000 Millipore AB5539 Parvalbumin (PV) mouse 1:1000 Swant PV235 Pou4f1 (Brn-3a) mouse 1:50 Millipore MAB1585 RFP rabbit 1:1000 Bio Vision 3993-100 Syt2 (Znp-1) mouse 1:1000 ZIRC AB_10013783 pure tones (10–14 kHz) lasting up to 1 min, during the P21–P28 and P35– P40 periods.

Techniques: Knock-Out, Staining, Control

Journal: Theranostics

Article Title: Rbm24/Notch1 signaling regulates adult neurogenesis in the subventricular zone and mediates Parkinson-associated olfactory dysfunction.

doi: 10.7150/thno.96045

Figure Lengend Snippet: Figure 3. Rbm24 deficiency impedes adult neurogenesis in the OB. (A) Representative images of BrdU/NeuN co-staining in the OB from 3-month-old CTL and UKO mice. Scale bar, 100 μm. (B) Quantification of BrdU+ and BrdU+/NeuN+ cells in the OB from 3-month-old CTL and UKO mice (n = 4 mice for each group). (C-D) Representative images and quantification of TUNEL+ cells in the OB from 3-month-old CTL and UKO mice (n = 4 mice for each group). Scale bar, 100 μm. (E-I) Representative images and quantification of TH+ (E, G), CalB+ (F, G), CalR+ (H, I) cells in the OB from 4-month-old CTL and UKO mice 60 days after TAM administration (n = 4 mice for each group). Scale bar, 100 μm. (J) Representative traces of sIPSC of M/Ts in the OB from 4-month-old CTL and UKO mice. Scale bar, 40 pA and 20 s. (K) Cumulative curves of sIPSC interevent interval of M/Ts in the OB from 4-month-old CTL and UKO mice. (L) Quantification of sIPSC frequency of M/Ts in (J) (n = 4 mice for each group). (M) Cumulative curves of sIPSC amplitude of M/Ts in the OB from 4-month-old CTL and UKO mice. (N) Quantification of sIPSC amplitude of M/Ts in (J) (n = 4 mice for each group). Data are presented as mean ± SEM. *p < 0.05; **p < 0.01; n.s., not significant. OB: olfactory bulb; GL: glomerular layer; GCL: granule cell layer.

Article Snippet: Slices (20 μm thickness) from the SVZ, RMS, and OB were collected and incubated overnight at 4 °C with primary antibodies: Rbm24 (1:200, ab94567, Abcam, UK), GFAP (1:500, 3670S, Cell Signaling Technology, USA), DCX (1:300, sc271390, Santa Cruz, USA), BrdU (1:250, ab6326, Abcam, UK), Caspase-3 (1:100, T40044, Abmart, China), NeuN (1:500, ab177487, Abcam, UK), TH (1:200, ab1542, Sigma, USA), CalR (1:100, sc365956, Santa Cruz, USA), CalB (1:200, 13176S, Cell Signaling Technology, USA), Notch1 (1:100, 3447S, Cell Signaling Technology, USA), and GFP (1:300, ab290, Abcam, UK).

Techniques: Staining, TUNEL Assay